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hkl hrigi  (InvivoGen)


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    Structured Review

    InvivoGen hkl hrigi
    Hkl Hrigi, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hkl+hrigi/HEK-Lucia+RIG-I+Cells/pm41603157-242-10-7
    Average 94 stars, based on 27 article reviews
    hkl hrigi - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Luciferase:

    Article Title: The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor
    Article Snippet: The pcDNA TM 3.1(+) empty vector (Life Technologies) was used as a control for cDNA transfection. .. HEK-293T cells (cat. number 103, NIH AIDS Research and Reference Reagent Program) and Lucia luciferase reporter HEK-293 cells expressing human RIG-I, HEK-LuciaTM RIG-I, (hkl-hrigi, InvivoGen, San Diego, CA, USA) were grown at 37 °C in a humidified atmosphere with 5% CO 2 in DMEM (Lonza, Verviers, Belgium) supplemented with 10% foetal calf serum (FCS) (Lonza), 1% L-glutamine and 1% penicillin–streptomycin (Lonza, Basel, Switzerland). .. The cells were harvested and passaged every 3 days using trypsin-EDTA (L0930-100) (Biowest, Lakewood Ranch, FL, USA) or Versene 1× (15040-066; Gibco Chemicals, Thermo Fisher Scientific, MA, USA).

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Article Title: Non‐Targeting shRNA‐Encoded Plasmid DNA Enhances Protective Immunity Through RIDD‐RIG‐I Signaling Pathway in the Zika Virus Animal Model
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL‐3216) were used for siRNA transfection to assess protein expression of RIDD‐related genes. .. The RIG‐I reporter cell line, HEK‐Lucia RIG‐I (InvivoGen; Cat. # hkl‐hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG‐I pathway activation. .. The TLR3‐IFN‐β‐Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung‐Hsien Chuang, NHRI, Taiwan) [ ], was generated by stable transfection with a TLR3 expression vector and an IFN‐β promoter‐driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Expressing:

    Article Title: The ZIKV NS5 Protein Aberrantly Alters the Tubulin Cytoskeleton, Induces the Accumulation of Autophagic p62 and Affects IFN Production: HDAC6 Has Emerged as an Anti-NS5/ZIKV Factor
    Article Snippet: The pcDNA TM 3.1(+) empty vector (Life Technologies) was used as a control for cDNA transfection. .. HEK-293T cells (cat. number 103, NIH AIDS Research and Reference Reagent Program) and Lucia luciferase reporter HEK-293 cells expressing human RIG-I, HEK-LuciaTM RIG-I, (hkl-hrigi, InvivoGen, San Diego, CA, USA) were grown at 37 °C in a humidified atmosphere with 5% CO 2 in DMEM (Lonza, Verviers, Belgium) supplemented with 10% foetal calf serum (FCS) (Lonza), 1% L-glutamine and 1% penicillin–streptomycin (Lonza, Basel, Switzerland). .. The cells were harvested and passaged every 3 days using trypsin-EDTA (L0930-100) (Biowest, Lakewood Ranch, FL, USA) or Versene 1× (15040-066; Gibco Chemicals, Thermo Fisher Scientific, MA, USA).

    Activity Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Article Title: Non‐Targeting shRNA‐Encoded Plasmid DNA Enhances Protective Immunity Through RIDD‐RIG‐I Signaling Pathway in the Zika Virus Animal Model
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL‐3216) were used for siRNA transfection to assess protein expression of RIDD‐related genes. .. The RIG‐I reporter cell line, HEK‐Lucia RIG‐I (InvivoGen; Cat. # hkl‐hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG‐I pathway activation. .. The TLR3‐IFN‐β‐Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung‐Hsien Chuang, NHRI, Taiwan) [ ], was generated by stable transfection with a TLR3 expression vector and an IFN‐β promoter‐driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Transfection:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Article Title: Non‐Targeting shRNA‐Encoded Plasmid DNA Enhances Protective Immunity Through RIDD‐RIG‐I Signaling Pathway in the Zika Virus Animal Model
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL‐3216) were used for siRNA transfection to assess protein expression of RIDD‐related genes. .. The RIG‐I reporter cell line, HEK‐Lucia RIG‐I (InvivoGen; Cat. # hkl‐hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG‐I pathway activation. .. The TLR3‐IFN‐β‐Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung‐Hsien Chuang, NHRI, Taiwan) [ ], was generated by stable transfection with a TLR3 expression vector and an IFN‐β promoter‐driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Activation Assay:

    Article Title: Non-Targeting shRNA-Encoded Plasmid DNA Enhances Protective Immunity Through RIDD-RIG-I Signaling Pathway in the Zika Virus Animal Model.
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were used for siRNA transfection to assess protein expression of RIDD-related genes. .. The RIG-I reporter cell line, HEK-Lucia RIG-I (InvivoGen; Cat. # hkl-hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG-I pathway activation. .. The TLR3-IFN- β-Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung-Hsien Chuang, NHRI, Taiwan) [ 29 ], was generated by stable transfection with a TLR3 expression vector and an IFN- β promoter-driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Article Title: Non‐Targeting shRNA‐Encoded Plasmid DNA Enhances Protective Immunity Through RIDD‐RIG‐I Signaling Pathway in the Zika Virus Animal Model
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, CRL‐3216) were used for siRNA transfection to assess protein expression of RIDD‐related genes. .. The RIG‐I reporter cell line, HEK‐Lucia RIG‐I (InvivoGen; Cat. # hkl‐hrigi), was used in luciferase activity assays following siRNA transfection to evaluate RIG‐I pathway activation. .. The TLR3‐IFN‐β‐Luc reporter cell line, derived from HEK293 cells (provided by Dr. Tsung‐Hsien Chuang, NHRI, Taiwan) [ ], was generated by stable transfection with a TLR3 expression vector and an IFN‐β promoter‐driven MetLuc reporter construct (Clontech Laboratories, Inc.).

    Derivative Assay:

    Article Title: Modulation of SF3B1 in the pre-mRNA spliceosome induces a RIG-I-dependent type I IFN response
    Article Snippet: B16F10, CT26, 4T1, and MC38 murine lines were purchased from American Type Culture Collection, expanded, confirmed to be mycoplasma free, and then frozen as aliquots. .. HEK reporter lines, A549-Dual, and the derived genetic KO reporter lines were purchased from Invivogen: HEK-Lucia Null (hkl-null), HEK-Lucia RIG-I (hkl-hrigi), A549-Dual (a549d-nfis), A549-Dual-RIG-I-KO (a549d-korigi), A549-Dual-MDA5-KO (a549d-komda5), A549-Dual-MAVS-KO (a549d-komavs), THP1-Dual (thpd-nfis), THP1-Dual-IRF3-KO (thpd-koirf3), and THP1-Dual-MAVS-KO (thpd-komavs). .. All reporter cell lines from Invivogen were maintained as recommended by the manufacturer.



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    mRNA generated by T7 RNAP variants had enhanced translation and reduced immunogenicity in vitro mRNA generated by the R632N and Q649L variants at 4 mM and 0.5 mM cap concentration was compared with mRNA generated by WT T7 RNAP for translation and immunogenicity in vitro . mRNAs were transfected to primary human hepatocytes to quantify (A) Cas9 expression and (B) GFP expression. HEK-Lucia RIG-I reporter cells were used to quantity immunogenicity of (C) Cas9 mRNA and (D) GFP mRNA. DC was 142-bp dsRNA as positive control and LC was lipofectamine as negative control. Cell studies were performed with three biological replicates for each condition. Data are presented as mean values, with error bars representing the standard deviation. GraphPad Prism 10.5.0 was used to perform unpaired t test to calculate significance ( p value).

    Journal: Molecular Therapy Advances

    Article Title: Engineered T7 RNA polymerase to improve mRNA capping efficiency and reduce dsRNA generation during in vitro transcription

    doi: 10.1016/j.omta.2026.201722

    Figure Lengend Snippet: mRNA generated by T7 RNAP variants had enhanced translation and reduced immunogenicity in vitro mRNA generated by the R632N and Q649L variants at 4 mM and 0.5 mM cap concentration was compared with mRNA generated by WT T7 RNAP for translation and immunogenicity in vitro . mRNAs were transfected to primary human hepatocytes to quantify (A) Cas9 expression and (B) GFP expression. HEK-Lucia RIG-I reporter cells were used to quantity immunogenicity of (C) Cas9 mRNA and (D) GFP mRNA. DC was 142-bp dsRNA as positive control and LC was lipofectamine as negative control. Cell studies were performed with three biological replicates for each condition. Data are presented as mean values, with error bars representing the standard deviation. GraphPad Prism 10.5.0 was used to perform unpaired t test to calculate significance ( p value).

    Article Snippet: HEK-Lucia RIG-I reporter cells (InvivoGen) were maintained in DMEM supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific), normocin (100 μg/mL), penicillin-streptomycin (100 U/mL), blasticidin (30 μg/mL), and zeocin (100 μg/mL).

    Techniques: Generated, Immunopeptidomics, In Vitro, Concentration Assay, Transfection, Expressing, Positive Control, Negative Control, Standard Deviation

    mRNA generated by T7 RNAP variants had enhanced translation and reduced immunogenicity in vivo Mice were dosed intravenously with Cas9 mRNA generated by the R632N and Q649L variants at 4 and 0.5 mM cap concentration. These were compared to mRNA produced by WT T7 RNAP to assess (A) Cas9 expression, (B) IFN-α, (C) IFN-β, and (D) IP-10. HEK-Lucia RIG-I reporter cells were used to quantify immunogenicity of (C) Cas9 mRNA and (D) GFP mRNA. Phosphate-buffered saline (PBS) was used as control. Data are presented as mean, with error bars representing the standard deviation ( n = 5 mice). GraphPad Prism 10.5.0 was used to perform unpaired t test to calculate significance ( p value).

    Journal: Molecular Therapy Advances

    Article Title: Engineered T7 RNA polymerase to improve mRNA capping efficiency and reduce dsRNA generation during in vitro transcription

    doi: 10.1016/j.omta.2026.201722

    Figure Lengend Snippet: mRNA generated by T7 RNAP variants had enhanced translation and reduced immunogenicity in vivo Mice were dosed intravenously with Cas9 mRNA generated by the R632N and Q649L variants at 4 and 0.5 mM cap concentration. These were compared to mRNA produced by WT T7 RNAP to assess (A) Cas9 expression, (B) IFN-α, (C) IFN-β, and (D) IP-10. HEK-Lucia RIG-I reporter cells were used to quantify immunogenicity of (C) Cas9 mRNA and (D) GFP mRNA. Phosphate-buffered saline (PBS) was used as control. Data are presented as mean, with error bars representing the standard deviation ( n = 5 mice). GraphPad Prism 10.5.0 was used to perform unpaired t test to calculate significance ( p value).

    Article Snippet: HEK-Lucia RIG-I reporter cells (InvivoGen) were maintained in DMEM supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific), normocin (100 μg/mL), penicillin-streptomycin (100 U/mL), blasticidin (30 μg/mL), and zeocin (100 μg/mL).

    Techniques: Generated, Immunopeptidomics, In Vivo, Concentration Assay, Produced, Expressing, Saline, Control, Standard Deviation